2.43
1.65
1.98
0.73
1.88
1.81
2.43
2.2 Stuthan àbhaisteach a thathar a’ cleachdadh ann an lùb calabrachaidh sgaoileadh mais mhóileciuil coimeasach: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ionnsramaidean agus uidheam
23.2
21.4
22.2
16.1
22.3
20.8
23.9
27.5
Gu h-iomlan, tha an co-roinn de amino-aigéid ann am bathar Sustar nas àirde na an co-roinn ann am bathar Zinpro.
Pàirt 8 Buaidhean cleachdaidh
Buaidh diofar thùsan de mhèinnirean lorg air coileanadh cinneasachaidh agus càileachd uighean chearcan breith anns an ùine breith anmoch
Pròiseas Riochdachaidh
Teicneòlas chelation cuimsichte
Teicneòlas emulsification rùsgadh
Teicneòlas spraeadh is tiormachaidh cuideam
Teicneòlas fuarachaidh is dì-taiseachaidh
Teicneòlas smachd àrainneachdail adhartach
Leas-phàipear A: Modhan airson sgaoileadh mais mhóileciuil coimeasach peptidean a dhearbhadh
Gabhail ris an inbhe: GB/T 22492-2008
1 Prionnsabal Deuchainn:
Chaidh a dhearbhadh le cromatagrafaidheachd sìolaidh gel àrd-choileanaidh. 'S e sin ri ràdh, a' cleachdadh lìonadh porous mar ìre statach, stèidhichte air an eadar-dhealachadh ann am meud mais moileciuil coimeasach nan co-phàirtean sampall airson dealachadh, air a lorg aig a' cheangal peptide den tonn-fhaid gabhail ultraviolet de 220nm, a' cleachdadh am bathar-bog giollachd dàta sònraichte airson sgaoileadh mais moileciuil coimeasach a dhearbhadh le cromatagrafaidheachd sìolaidh gel (ie, am bathar-bog GPC), chaidh na cromatograman agus an dàta aca a phròiseasadh, air an obrachadh a-mach gus meud mais moileciuil coimeasach a' pheaptaid pònairean soighe agus an raon sgaoilidh fhaighinn.
2. Ath-bheachdan
Bu chòir don uisge deuchainneach coinneachadh ri sònrachadh uisge àrd-sgoile ann an GB/T6682, agus bu chòir do luchd-reagent a thathar a’ cleachdadh, ach a-mhàin ann an ullachaidhean sònraichte, a bhith fìor-ghlan gu anailiseach.
2.1 Tha ath-bheachdan a’ gabhail a-steach acetonitrile (glan gu cròmatagrafaigeach), searbhag trifluoroacetic (glan gu cròmatagrafaigeach),
2.2 Stuthan àbhaisteach a thathar a’ cleachdadh ann an lùb calabrachaidh sgaoileadh mais mhóileciuil coimeasach: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Ionnsramaidean agus uidheam
3.1 Cromatagraf Leachtach Àrd-choileanaidh (HPLC): stèisean-obrach cromatagrafaidheachd no amalaiche le lorgaire UV agus bathar-bog giollachd dàta GPC.
3.2 Aonad sìolaidh is dì-ghasaidh falamh ìre gluasadach.
3.3 Cothromachadh dealanach: luach ceumnaichte 0.000 1g.
4 Ceumannan obrachaidh
4.1 Cumhaichean cròmatagrafaigeach agus deuchainnean atharrachaidh siostam (cumhaichean iomraidh)
- 4.1.1 Colbh cròmatagrafach: TSKgelG2000swxl300 mm × 7.8 mm (trast-thomhas a-staigh) no colbhan gel eile den aon seòrsa le coileanadh coltach ris a tha freagarrach airson pròtainean agus peptidean a dhearbhadh.
- 4.1.2 Ìre gluasadach: Acetonitrile + uisge + searbhag trifluoroacetic = 20 + 80 + 0.1.
- 4.1.3 Tonn-fhaid lorgaidh: 220 nm.
- 4.1.4 Ìre sruth: 0.5 mL/min.
- 4.1.5 Ùine lorgaidh: 30 mionaid.
- 4.1.6 Meud stealladh sampall: 20μL.
- 4.1.7 Teòthachd a’ cholbh: teòthachd an t-seòmair.
- 4.1.8 Gus am biodh an siostam cròmagrafach a’ coinneachadh ris na riatanasan lorg, chaidh a shònrachadh, fo na cumhaichean cròmagrafach gu h-àrd, nach biodh èifeachdas colbh cròmagrafach gel, i.e., an àireamh teòiridheach de phlàtaichean (N), nas lugha na 10000 air a thomhas a rèir mhullaichean an inbhe tripeptide (Glycine-Glycine-Glycine).
- 4.2 Riochdachadh lùban àbhaisteach mais mhóileciuil coimeasach
- Chaidh na fuasglaidhean àbhaisteach peptide mais mhóileciuil coimeasach eadar-dhealaichte gu h-àrd le dùmhlachd mais de 1 mg / mL ullachadh le bhith a’ maidseadh ìre gluasadach, gan measgachadh ann an co-mheas sònraichte, agus an uairsin gan sìoladh tro membran ìre organach le meud pore de 0.2 μm ~ 0.5 μm agus gan stealladh a-steach don sampall, agus an uairsin fhuaireadh cromatagraim nan inbhean. Fhuaireadh lùban calabrachaidh mais mhóileciuil coimeasach agus na co-aontaran aca le bhith a’ plotadh logarithm mais mhóileciuil coimeasach an aghaidh ùine gleidhidh no le ath-tharraing loidhneach.
4.3 Làimhseachadh sampall
Tomhais 10mg den sampall gu ceart ann am flasg tomhais-lìonaidh 10mL, cuir beagan den ìre gluasadach ris, crathadh le ultrasonaig airson 10 mionaidean, gus am bi an sampall air a sgaoileadh gu tur agus air a mheasgachadh, air a lagachadh leis an ìre gluasadach chun na sgèile, agus an uairsin air a shìoladh tro membran ìre organach le meud pore de 0.2μm ~ 0.5μm, agus chaidh an filtrate a sgrùdadh a rèir nan suidheachaidhean cròmagrafaidheachd ann an A.4.1.
- 5. Àireamhachadh sgaoileadh mais mhóileciuil coimeasach
- Às dèidh sgrùdadh a dhèanamh air fuasgladh an t-sampall a chaidh ullachadh ann an 4.3 fo na cumhaichean cromagrafaigeach ann an 4.1, gheibhear mais mhóileciuil coimeasach an t-sampall agus an raon sgaoilidh aige le bhith a’ cur dàta cromagrafaigeach an t-sampall a-steach don lùb calabrachaidh 4.2 le bathar-bog giollachd dàta GPC. Faodar sgaoileadh nan mais mhóileciuil coimeasach de na diofar pheaptidean obrachadh a-mach leis an dòigh àbhaisteachaidh air farsaingeachd nam bàrr, a rèir na foirmle: X=A/A iomlan × 100
- Anns an fhoirmle: X - An co-mheas mais de peptide mais mhóileciuil coimeasach anns a’ peptide iomlan san sampall, %;
- A - Raon as àirde de peptide le mais mhóileciuil coimeasach;
- Iomlan A - suim raointean nam mullaichean de gach peptide le mais mhóileciuil coimeasach, air a thomhas gu aon àite deicheach.
- 6 Ath-aithris
- Cha bu chòir an diofar iomlan eadar dà dhearbhadh neo-eisimeileach a gheibhear fo chumhachan ath-aithris a bhith nas àirde na 15% de chuibheasachd àireamhachd an dà dhearbhadh.
- Leas-phàipear B: Modhan airson Aimino-aigéid Shaor a dhearbhadh
- Gabhail ris an inbhe: Q/320205 KAVN05-2016
- 1.2 Ath-bheachdan agus stuthan
- searbhag aiseatach reòite: fìor-ghlan gu anailiseach
- searbhag perclòrach: 0.0500 mol/L
- Comharra: comharradh criostail bhiolet 0.1% (aigéad aiseatach reòthte)
- 2. Co-dhùnadh mu amino-aigéid shaora
Chaidh na sampallan a thiormachadh aig 80°C airson 1 uair a thìde.
Cuir an sampall ann an soitheach tioram gus am fuaraich e gu nàdarrach gu teòthachd an t-seòmair no gus am fuaraich e sìos gu teòthachd a ghabhas cleachdadh.Cuir timcheall air 0.1 g den sampall (cruinn gu 0.001 g) ann am flasg cònúil tioram 250 mL.Rach air adhart gu sgiobalta chun ath cheum gus casg a chuir air an sampall bho bhith a’ gabhail a-steach taiseachd àrainneachdailCuir 25 mL de dh’aigéad aiseatach reòthte ris agus measgaich gu math airson gun a bhith nas fhaide na 5 mionaidean.Cuir 2 dhiog de chomharradh criostail violet risTìtrate le fuasgladh titration àbhaisteach 0.0500 mol / L (±0.001) de dh’aigéad perclòrach gus an atharraich an fhuasgladh bho phurpaidh chun a’ phuing crìochnachaidh.
Clàraich meud an fhuasglaidh àbhaisteach a chaidh a chaitheamh.
- Dèan an deuchainn bàn aig an aon àm.
- 3. Àireamhachadh agus toraidhean
- Tha susbaint an amino-aigéid shaor X anns an ath-ghineadair air a chur an cèill mar bhloigh mais (%) agus air a thomhas a rèir na foirmle: X = C × (V1-V0) × 0.1445/M × 100%, san fhoirmle seo:
- C - Dùmhlachd fuasgladh searbhag perclòrach àbhaisteach ann am mòlan gach liotar (mol/L)
- V1 - Meud a thathar a’ cleachdadh airson titreachadh shamhlaichean le fuasgladh searbhag perclòrach àbhaisteach, ann am milliliters (mL).
- Vo - Meud a chaidh a chleachdadh airson titration bàn le fuasgladh searbhag perclòrach àbhaisteach, ann am milliliters (mL);
M - Mais an t-sampall, ann an graman (g).
| 0.1445: Tomad cuibheasach amino-aigéid co-ionann ri 1.00 mL de fhuasgladh searbhag perclòrach àbhaisteach [c (HClO4) = 1.000 mol / L]. | 4.2.3 Fuasgladh titration àbhaisteach cerium sulfate: dùmhlachd c [Ce (SO4) 2] = 0.1 mol/L, air ullachadh a rèir GB/T601. | |
| Gabhail ri inbhean: Q/70920556 71-2024 | 1. Prionnsabal dearbhaidh (Fe mar eisimpleir) | Tha solubhail glè ìosal aig co-thàthaidhean iarainn amino-aigéid ann an ethanol gun uisge agus tha ianan meatailt saor solubhail ann an ethanol gun uisge, chaidh an diofar ann an solubhail eadar an dà rud ann an ethanol gun uisge a chleachdadh gus an ìre chelation de cho-thàthaidhean iarainn amino-aigéid a dhearbhadh. |
| Anns an fhoirmle: V1 - meud fuasgladh àbhaisteach cerium sulfate a chaidh a chaitheamh airson titration an fhuasglaidh deuchainn, mL; | Ethanol gun uisge; tha an còrr mar an ceudna ri clàs 4.5.2 ann an GB/T 27983-2011. | 3. Ceumannan anailis |
| Dèan dà dheuchainn aig an aon àm. Tomhais 0.1g den sampall air a thiormachadh aig 103±2℃ airson 1 uair, le cruinneas gu 0.0001g, cuir 100mL de ethanol gun uisge ris gus a sgaoileadh, sìolaich, sìolaich an còrr air a nighe le 100mL de ethanol gun uisge airson co-dhiù trì tursan, an uairsin gluais an còrr gu flasg cònail 250mL, cuir 10mL de fhuasgladh searbhag sulfarach ris a rèir clàs 4.5.3 ann an GB/T27983-2011, agus an uairsin dèan na ceumannan a leanas a rèir clàs 4.5.3 “Teas gus a sgaoileadh agus an uairsin leig leis fuarachadh” ann an GB/T27983-2011. Dèan an deuchainn bàn aig an aon àm. | 4. Co-dhùnadh susbaint iomlan iarainn | 4.1 Tha am prionnsabal dearbhaidh mar an ceudna ri clàs 4.4.1 ann an GB/T 21996-2008. |
4.2. Ath-bheachdan & Fuasglaidhean
| 4.2.1 Aigéad measgaichte: Cuir 150mL de dh’aigéad sulfarach agus 150mL de dh’aigéad fosfarach ri 700mL de dh’uisge agus measgaich gu math. | 4.2.2 Fuasgladh comharraiche sodium diphenylamine sulfonate: 5g/L, air ullachadh a rèir GB/T603. | 4.2.3 Fuasgladh titration àbhaisteach cerium sulfate: dùmhlachd c [Ce (SO4) 2] = 0.1 mol/L, air ullachadh a rèir GB/T601. | |
| 4.3 Ceumannan anailis | Dèan dà dheuchainn aig an aon àm. Tomhais 0.1g den sampall, ceart gu 020001g, cuir ann am flasg cònail 250mL e, cuir 10mL de dh’aigéad measgaichte ris, às dèidh a sgaoileadh, cuir 30ml de dh’uisge agus 4 boinneagan de fhuasgladh comharraiche sodium dianiline sulfonate ris, agus an uairsin dèan na ceumannan a leanas a rèir clàs 4.4.2 ann an GB/T21996-2008. Dèan an deuchainn bàn aig an aon àm. | 4.4 Riochdachadh nan toraidhean | Chaidh susbaint iomlan iarainn X1 de na co-thàthaidhean iarainn amino-aigéid a thaobh meud an iarainn, an luach air a chur an cèill ann an %, obrachadh a-mach a rèir foirmle (1): |
| X1=(V-V0)×C×M×10-3×100 | V0 - fuasgladh àbhaisteach cerium sulfate air a chleachdadh airson titration fuasgladh bàn, mL; | V0 - fuasgladh àbhaisteach cerium sulfate air a chleachdadh airson titration fuasgladh bàn, mL; | C - Dùmhlachd fìor fhuasgladh àbhaisteach cerium sulfate, mol/L5. Àireamhachadh susbaint iarainn ann an chelatesChaidh susbaint an iarainn X2 anns a’ chelate a thaobh meud na h-iarainn, an luach air a chur an cèill ann an %, obrachadh a-mach a rèir na foirmle: x2 = ((V1-V2) × C × 0.05585)/m1 × 100 |
| Anns an fhoirmle: V1 - meud fuasgladh àbhaisteach cerium sulfate a chaidh a chaitheamh airson titration an fhuasglaidh deuchainn, mL; | V2 - fuasgladh àbhaisteach cerium sulfate air a chleachdadh airson titration fuasgladh bàn, mL;nom1 - Mais an t-sampall, g. Gabh a’ chuibheasachd àireamhachd de thoraidhean an dearbhaidh co-shìnte mar thoraidhean an dearbhaidh, agus chan eil an diofar iomlan de thoraidhean an dearbhaidh co-shìnte nas motha na 0.3%. | 0.05585 - mais iarainn ferrous air a chur an cèill ann an graman co-ionann ri 1.00 mL de fhuasgladh àbhaisteach cerium sulfate C[Ce(SO4)2.4H20] = 1.000 mol/L.nom1 - Mais an t-sampall, g. Gabh a’ chuibheasachd àireamhachd de thoraidhean an dearbhaidh co-shìnte mar thoraidhean an dearbhaidh, agus chan eil an diofar iomlan de thoraidhean an dearbhaidh co-shìnte nas motha na 0.3%. | 6. Àireamhachadh ìre chelationÌre cealachaidh X3, an luach air a chur an cèill ann an %, X3 = X2/X1 × 100Leas-phàipear C: Modhan airson ìre chelation Zinpro a dhearbhadh |
Gabhail ris an inbhe: Q/320205 KAVNO7-2016
1. Ath-bheachdan agus stuthan
a) searbhag aiseatach reòite: fìor-ghlan gu anailiseach; b) searbhag phearclòrach: 0.0500mol/L; c) Comharra: comharra criostail bhiolet 0.1% (searbhag aiseatach reòite)
2. Co-dhùnadh mu amino-aigéid shaora
2.1 Chaidh na sampallan a thiormachadh aig 80°C airson 1 uair a thìde.
2.2 Cuir an sampall ann an soitheach tioram gus fuarachadh gu nàdarrach gu teòthachd an t-seòmair no fuarachadh sìos gu teòthachd a ghabhas cleachdadh.
2.3 Tomhais timcheall air 0.1 g den sampall (cruinn gu 0.001 g) a-steach do fhlasg cònaigeach tioram 250 mL
2.4 Rach air adhart gu sgiobalta chun ath cheum gus casg a chuir air an sampall bho bhith a’ gabhail a-steach taiseachd na h-àrainneachd.
2.5 Cuir 25mL de dh’aigéad aiseatach reòthte ris agus measgaich gu math airson gun a bhith nas fhaide na 5 mionaidean.
2.6 Cuir 2 dhiog de chomharradh criostail violet ris.
2.7 Tìtreadh le fuasgladh tiotraidh àbhaisteach 0.0500mol/L (±0.001) de dh’aigéad perclòrach gus an atharraich an fhuasgladh bho phurpaidh gu uaine airson 15 diogan gun dath atharrachadh mar a’ cheann thall.
2.8 Clàraich meud an fhuasglaidh àbhaisteach a chaidh ithe.
2.9 Dèan an deuchainn bàn aig an aon àm.
- 3. Àireamhachadh agus toraidhean
- Catalanach
- Physicochemical parameters
V1 - Meud a thathar a’ cleachdadh airson titreachadh shamhlaichean le fuasgladh searbhag perclòrach àbhaisteach, ann am milliliters (mL).
Vo - Meud a chaidh a chleachdadh airson titration bàn le fuasgladh searbhag perclòrach àbhaisteach, ann am milliliters (mL);
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Seòladh: Àir. 147 Rathad Qingpu, Baile Shouan, Siorrachd Pujiang, Cathair Chengdu, Roinn Sichuan, Sìona
Fòn: 86-18880477902
Toraidhean
mèinnirean lorg neo-organach
- Mèinnirean lorg organach
- Swahilieach
- Seirbheis gnàthaichte
- Ceanglaichean luath
Pròifil Companaidh
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Briog airson ceist | © Dlighe-sgrìobhaidh - 2010-2025: Gach còir glèidhte. | Mapa-làraich PRÌOMH RANNSACHADH Fòn |
| Fòn | 86-18880477902 | Iabhaininnseach | Post-d |
| 8618880477902 | Sìneach | Frangach | |
| Bird | Sìneach | Frangach | Gearmailteach Spàinneach |
| Aquatic animals | Iapanach | Corèanach | Arabach Grèigeach |
| Turcach | Eadailteach | ||
| Ruminant animal g/head day | January 0.75 | And-Innseach Afrikaanach Suaineach |
Pòlainneach
- Basgach
- Catalanach
- Physicochemical parameters
Indeach
Lao
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Bulgàrianach
- Cebuano
- This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
- The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
- Croatianach
Duitseach
| Application object | Urdu Bhiet-Namach | Content in full-value feed (mg/kg) | Efficacy |
| Gujarati | Haitianach | Hausa | Cìniarwanda Hmong Hungàrianach |
| Piglets and fattening pigs | Igbo | Iabhaininnseach | Kannada Chmer Curdach |
| Kyrgyz | Laidinn | ||
| Bird | 300～400 | 45～60 | Macedonianach Malaidheach Malayalamach |
| Aquatic animals | 200～300 | 30～45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
Lochlannach
- Pashto
- Appearance: brownish-yellow granules
- Physicochemical parameters
Serbianach
Sesotho
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
Shona
Sindeach
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
Swahilieach
Tajik
Tamileach
Telugach
Thaidheach
| Application object | Urdu Bhiet-Namach | Content in full-value feed (mg/kg) | Efficacy |
| Iùdhais | Iorùba | Zulu | Cìniarwanda Oriyach Turkmen |
| Uyghur | 250～400 | 37.5～60 | 1. Improving the immunity of piglets, reducing diarrhea and mortality; 2. Improving palatability, increasing feed intake, increasing growth rate and improving feed conversion; 3. Make the pig coat bright and improve the carcass quality and meat quality. |
| Bird | 300～400 | 45～60 | 1. Improve feather glossiness; 2. improve the laying rate, fertilization rate and hatching rate of breeding eggs, and strengthen the coloring ability of egg yolk; 3. Improve anti-stress ability and reduce mortality; 4. Improve feed conversion and increase growth rate. |
| Aquatic animals | January 300 | 45 | 1. Promote growth, improve feed conversion; 2. Improve anti-stress abolity, reduce morbidity and mortality. |
| Ruminant animal g/head day | 2.4 | 1. Improve milk yield, prevent mastitis and foof rot, and reduce somatic cell content in milk; 2. Promote growth, improve feed conversion and improve meat quality. |
4. Manganese Amino Acid Chelate Feed Grade
- Product Name: Manganese Amino Acid Chelate Feed Grade
- Appearance: brownish-yellow granules
- Physicochemical parameters
a) Mn: ≥ 10.0%
b) Total amino acids: ≥ 19.5%
c) Chelation rate: ≥ 95%
d) Arsenic: ≤ 2 mg/kg
e) Lead: ≤ 5 mg/kg
f) Cadmium: ≤ 5 mg/kg
g) Moisture content: ≤ 5.0%
h) Fineness: All particles pass through 20 mesh, with a main particle size of 60-80 mesh
n=0, 1,2,...indicates chelated manganese for dipeptides, tripeptides, and tetrapeptides
Characteristics of Manganese Amino Acid Chelate Feed Grade
This product is an all-organic trace mineral chelated by a special chelating proces with pure plant enzymatic small molecule peptides as chelating substrates and trace elements;
This product is chemically stable and can significantly reduce its damage to vitamins and fats, etc. The use of this product is conducive to improving feed quality;
The product is absorbed through small peptide and amino acid pathways, reducing the competition and antagonism with other trace elements, and has the best bio-absorption and utilization rate;
The product can improve the growth rate, improve feed conversion and health status significantly; and improve the laying rate, hatching rate and healthy chick rate of breeding poultry obviously;
Manganese is necessary for bone growth and connective tissue maintenance. It is closely related to many enzymes; and participates in carbohydrate, fat and protein metabolism, reproduction and immune response.
Usage and Efficacy of Manganese Amino Acid Chelate Feed Grade
| Application object | Suggested dosage (g/t full-value material) | Content in full-value feed (mg/kg) | Efficacy |
| Breeding pig | 200～300 | 30～45 | 1. Promote the normal development of sexual organs and improve sperm motility; 2. Improve the reproductive capacity of breeding pigs and reduce reproductive obstacles. |
| Piglets and fattening pigs | 100～250 | 15～37.5 | 1. It is beneficial to improve immune functions, and improve anti-stress ability and disease resistance; 2. Promote growth and improve feed conversion significantly; 3. Improve meat color and quality, and improve lean meat percentage. |
| Bird | 250～350 | 37.5～52.5 | 1. Improve anti-stress ability and reduce mortality; 2. Improve laying rate, fertilization rate and hatching rate of breeding eggs, improve eggshell quality and reduce shell breaking rate; 3. Promote bone growth and reduce the incidence of leg diseases. |
| Aquatic animals | 100～200 | 15～30 | 1. Promote growth and improve its anti-stress ability and disease resistance; 2. Improve sperm motility and hatching rate of fertilized eggs. |
| Ruminant animal g/head day | Cattle 1.25 | 1. Prevent fatty acid synthesis disorder and bone tissue damage; 2. Improve reproductive capacity, prevent abortion and postpartum paralysis of female animals, reduce the mortality of calves and lambs, and increase the newborn weight of young animals. | |
| Goat 0.25 |
Part 6 FAB of Small Peptide-mineral Chelates
| S/N | F: Functional attributes | A: Competitive differences | B: Benefits brought by competitive differences to users |
| 1.52 | Selectivity control of raw materials | Select pure plant enzymatic hydrolysis of small peptides | High biological safety, avoiding cannibalism |
| 2 | Directional digestion technology for double protein biological enzyme | High proportion of small molecular peptides | More "targets", which are not easy to saturation, with high biological activity and better stability |
| 3 | Advanced pressure spray & drying technology | Granular product, with uniform particle size, better fluidity, not easy to absorb moisture | Ensure easy to use, more uniform mixing in complete feed |
| Low water content (≤ 5%), which greatly reduces the influence caused by vitamins and enzyme preparations | Improve the stability of feed products | ||
| 4 | Advanced production control technology | Totally enclosed process, high degree of automatic control | Safe and stable quality |
| 5 | Advanced quality control technology | Establish and improve scientific and advanced analytical methods and control means for detecting factors affecting product quality, such as acid-soluble protein, molecular weight distribution, amino acids and chelating rate | Ensure quality, ensure efficiency and improve efficiency |
Part 7 Competitor Comparison
Standard VS Standard
Comparison of peptide distribution and chelation rate of products
| Sustar's products | Proportion of small peptides（180-500） | Zinpro's products | Proportion of small peptides（180-500） |
| AA-Cu | ≥74% | AVAILA-Cu | 78% |
| AA-Fe | ≥48% | AVAILA-Fe | 59% |
| AA-Mn | ≥33% | AVAILA-Mn | 53% |
| AA-Zn | ≥37% | AVAILA-Zn | 56% |
| Sustar's products | Chelation rate | Zinpro's products | Chelation rate |
| AA-Cu | 94.8% | AVAILA-Cu | 94.8% |
| AA-Fe | 95.3% | AVAILA-Fe | 93.5% |
| AA-Mn | 94.6% | AVAILA-Mn | 94.6% |
| AA-Zn | 97.7% | AVAILA-Zn | 90.6% |
The ratio of small peptides of Sustar is slightly lower than that of Zinpro, and the chelation rate of Sustar's products is slightly higher than that of Zinpro's products.
Comparison of the content of 17 amino acids in different products
| Name of amino acids | Sustar's Copper Amino Acid Chelate Feed Grade | Zinpro's AVAILA copper | Sustar's Ferrous Amino Acid C helate Feed Grade | Zinpro's AVAILA iron | Sustar's Manganese Amino Acid Chelate Feed Grade | Zinpro's AVAILA manganese | Sustar's Zinc Amino Acid Chelate Feed Grade | Zinpro's AVAILA zinc |
| aspartic acid (%) | 1.88 | 0.72 | 1.50 | 0.56 | 1.78 | 1.47 | 1.80 | 2.09 |
| glutamic acid (%) | 4.08 | 6.03 | 4.23 | 5.52 | 4.22 | 5.01 | 4.35 | 3.19 |
| Serine (%) | 0.86 | 0.41 | 1.08 | 0.19 | 1.05 | 0.91 | 1.03 | 2.81 |
| Histidine (%) | 0.56 | 0.00 | 0.68 | 0.13 | 0.64 | 0.42 | 0.61 | 0.00 |
| Glycine (%) | 1.96 | 4.07 | 1.34 | 2.49 | 1.21 | 0.55 | 1.32 | 2.69 |
| Threonine (%) | 0.81 | 0.00 | 1.16 | 0.00 | 0.88 | 0.59 | 1.24 | 1.11 |
| Arginine (%) | 1.05 | 0.78 | 1.05 | 0.29 | 1.43 | 0.54 | 1.20 | 1.89 |
| Alanine (%) | 2.85 | 1.52 | 2.33 | 0.93 | 2.40 | 1.74 | 2.42 | 1.68 |
| Tyrosinase (%) | 0.45 | 0.29 | 0.47 | 0.28 | 0.58 | 0.65 | 0.60 | 0.66 |
| Cystinol (%) | 0.00 | 0.00 | 0.09 | 0.00 | 0.11 | 0.00 | 0.09 | 0.00 |
| Valine (%) | 1.45 | 1.14 | 1.31 | 0.42 | 1.20 | 1.03 | 1.32 | 2.62 |
| Methionine (%) | 0.35 | 0.27 | 0.72 | 0.65 | 0.67 | 0.43 | January 0.75 | 0.44 |
| Phenylalanine (%) | 0.79 | 0.41 | 0.82 | 0.56 | 0.70 | 1.22 | 0.86 | 1.37 |
| Isoleucine (%) | 0.87 | 0.55 | 0.83 | 0.33 | 0.86 | 0.83 | 0.87 | 1.32 |
| Leucine (%) | 2.16 | 0.90 | 2.00 | 1.43 | 1.84 | 3.29 | 2.19 | 2.20 |
| Lysine (%) | 0.67 | 2.67 | 0.62 | 1.65 | 0.81 | 0.29 | 0.79 | 0.62 |
| Proline (%) | 2.43 | 1.65 | 1.98 | 0.73 | 1.88 | 1.81 | 2.43 | 2.78 |
| Total amino acids (%) | 23.2 | 21.4 | 22.2 | 16.1 | 22.3 | 20.8 | 23.9 | 27.5 |
Overall, the proportion of amino acids in Sustar's products is higher than that in Zinpro's products.
Part 8 Effects of use
Effects of different sources of trace minerals on the production performance and egg quality of laying hens in the late laying period
Production Process
- Targeted chelation technology
- Shear emulsification technology
- Pressure spray & drying technology
- Refrigeration & dehumidification technology
- Advanced environmental control technology
Appendix A: Methods for the Determination of relative molecular mass distribution of peptides
Adoption of standard: GB/T 22492-2008
1 Test Principle:
It was determined by high performance gel filtration chromatography. That is to say, using porous filler as stationary phase, based on the difference in the relative molecular mass size of the sample components for separation, detected at the peptide bond of the ultraviolet absorption wavelength of 220nm, using the dedicated data processing software for the determination of relative molecular mass distribution by gel filtration chromatography (i.e., the GPC software), the chromatograms and their data were processed, calculated to get the size of the relative molecular mass of the soybean peptide and the distribution range.
2. Reagents
The experimental water should meet the specification of secondary water in GB/T6682, the use of reagents, except for special provisions, are analytically pure.
2.1 Reagents include acetonitrile (chromatographically pure), trifluoroacetic acid (chromatographically pure),
2.2 Standard substances used in the calibration curve of relative molecular mass distribution: insulin, mycopeptides, glycine-glycine-tyrosine-arginine, glycine-glycine-glycine
3 Instrument and equipment
3.1 High Performance Liquid Chromatograph (HPLC): a chromatographic workstation or integrator with a UV detector and GPC data processing software.
3.2 Mobile phase vacuum filtration and degassing unit.
3.3 Electronic balance: graduated value 0.000 1g.
4 Operating steps
4.1 Chromatographic conditions and system adaptation experiments (reference conditions)
4.1.1 Chromatographic column: TSKgelG2000swxl300 mm×7.8 mm (inner diameter) or other gel columns of the same type with similar performance suitable for the determination of proteins and peptides.
4.1.2 Mobile phase: Acetonitrile + water + trifluoroacetic acid = 20 + 80 + 0.1.
4.1.3 Detection wavelength: 220 nm.
4.1.4 Flow rate: 0.5 mL/min.
4.1.5 Detection time: 30 min.
4.1.6 Sample injection volume: 20μL.
4.1.7 Column temperature: room temperature.
4.1.8 In order to make the chromatographic system meet the detection requirements, it was stipulated that under the above chromatographic conditions, the gel chromatographic column efficiency, i.e., the theoretical number of plates (N), was not less than 10000 calculated on the basis of the peaks of the tripeptide standard (Glycine-Glycine-Glycine).
4.2 Production of relative molecular mass standard curves
The above different relative molecular mass peptide standard solutions with a mass concentration of 1 mg / mL were prepared by mobile phase matching, mixed in a certain proportion, and then filtered through an organic phase membrane with the pore size of 0.2 μm~0.5 μm and injected into the sample, and then the chromatograms of the standards were obtained. Relative molecular mass calibration curves and their equations were obtained by plotting the logarithm of relative molecular mass against retention time or by linear regression.
4.3 Sample treatment
Accurately weigh 10mg of sample in a 10mL volumetric flask, add a little mobile phase, ultrasonic shaking for 10min, so that the sample is fully dissolved and mixed, diluted with mobile phase to the scale, and then filtered through an organic phase membrane with a pore size of 0.2μm~0.5μm, and the filtrate was analyzed according to the chromatographic conditions in A.4.1.
5. Calculation of relative molecular mass distribution
After analyzing the sample solution prepared in 4.3 under the chromatographic conditions of 4.1, the relative molecular mass of the sample and its distribution range can be obtained by substituting the chromatographic data of the sample into the calibration curve 4.2 with GPC data processing software. The distribution of the relative molecular masses of the different peptides can be calculated by the peak area normalization method, according to the formula: X=A/A total×100
In the formula: X - The mass fraction of a relative molecular mass peptide in the total peptide in the sample, %;
A - Peak area of a relative molecular mass peptide;
Total A - the sum of the peak areas of each relative molecular mass peptide, calculated to one decimal place.
6 Repeatability
The absolute difference between two independent determinations obtained under conditions of repeatability shall not exceed 15% of the arithmetic mean of the two determinations.
Appendix B: Methods for the Determination of Free Amino Acids
Adoption of standard: Q/320205 KAVN05-2016
1.2 Reagents and materials
Glacial acetic acid: analytically pure
Perchloric acid: 0.0500 mol/L
Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
The samples were dried at 80°C for 1 hour.
Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask.
Quickly proceed to the next step to avoid the sample from absorbing ambient moisture
Add 25 mL of glacial acetic acid and mix well for no more than 5 min.
Add 2 drops of crystal violet indicator
Titrate with 0.0500 mol / L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to the end point.
Record the volume of standard solution consumed.
Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%) and is calculated according to the formula: X = C × (V1-V0) × 0.1445/M × 100%, in tne formula:
C - Concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445: Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
Appendix C: Methods for the Determination of Sustar's chelation rate
Adoption of standards: Q/70920556 71-2024
1. Determination principle (Fe as an example)
Amino acid iron complexes have very low solubility in anhydrous ethanol and free metal ions are soluble in anhydrous ethanol, the difference in solubility between the two in anhydrous ethanol was utilized to determine the chelation rate of amino acid iron complexes.
2. Reagents & Solutions
Anhydrous ethanol; the rest is the same as clause 4.5.2 in GB/T 27983-2011.
3. Steps of analysis
Do two trials in parallel. Weigh 0.1g of the sample dried at 103±2℃ for 1 hour, accurate to 0.0001g, add 100mL of anhydrous ethanol to dissolve, filter, filter residue washed with 100mL of anhydrous ethanol for at least three times, then transfer the residue into a 250mL conical flask, add 10mL of sulfuric acid solution according to clause 4.5.3 in GB/T27983-2011, and then perform the following steps according to clause 4.5.3 “Heat to dissolve and then let cool” in GB/T27983-2011. Carry out the blank test at the same time.
4. Determination of total iron content
4.1 The principle of determination is the same as clause 4.4.1 in GB/T 21996-2008.
4.2. Reagents & Solutions
4.2.1 Mixed acid: Add 150mL of sulfuric acid and 150mL of phosphoric acid to 700mL of water and mix well.
4.2.2 Sodium diphenylamine sulfonate indicator solution: 5g/L, prepared according to GB/T603.
4.2.3 Cerium sulfate standard titration solution: concentration c [Ce (SO4) 2] = 0.1 mol/L, prepared according to GB/T601.
4.3 Steps of analysis
Do two trials in parallel. Weigh 0.1g of sample, accurate to 020001g, place in a 250mL conical flask, add 10mL of mixed acid, after dissolution, add 30ml of water and 4 drops of sodium dianiline sulfonate indicator solution, and then perform the following steps according to clause 4.4.2 in GB/T21996-2008. Carry out the blank test at the same time.
4.4 Representation of results
The total iron content X1 of the amino acid iron complexes in terms of mass fraction of iron, the value expressed in %, was calculated according to formula (1):
X1=(V-V0)×C×M×10-3×100
In the formula: V - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V0 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L
5. Calculation of iron content in chelates
The iron content X2 in the chelate in terms of the mass fraction of iron, the value expressed in %, was calculated according to the formula: x2 = ((V1-V2) × C × 0.05585)/m1 × 100
In the formula: V1 - volume of cerium sulfate standard solution consumed for titration of test solution, mL;
V2 - cerium sulfate standard solution consumed for titration of blank solution, mL;
C - Actual concentration of cerium sulfate standard solution, mol/L;
0.05585 - mass of ferrous iron expressed in grams equivalent to 1.00 mL of cerium sulfate standard solution C[Ce(SO4)2.4H20] = 1.000 mol/L.
m1-Mass of the sample, g. Take the arithmetic mean of the parallel determination results as the determination results, and the absolute difference of the parallel determination results is not more than 0.3%.
6. Calculation of chelation rate
Chelation rate X3, the value expressed in %, X3 = X2/X1 × 100
Appendix C: Methods for the Determination of Zinpro's chelation rate
Adoption of standard: Q/320205 KAVNO7-2016
1. Reagents and materials
a) Glacial acetic acid: analytically pure; b) Perchloric acid: 0.0500mol/L; c) Indicator: 0.1% crystal violet indicator (glacial acetic acid)
2. Determination of free amino acids
2.1 The samples were dried at 80°C for 1 hour.
2.2 Place the sample in a dry container to cool naturally to room temperature or cool down to a usable temperature.
2.3 Weigh approximately 0.1 g of sample (accurate to 0.001 g) into a 250 mL dry conical flask
2.4 Quickly proceed to the next step to avoid the sample from absorbing ambient moisture.
2.5 Add 25mL of glacial acetic acid and mix well for no more than 5min.
2.6 Add 2 drops of crystal violet indicator.
2.7 Titrate with 0.0500mol/L (±0.001) standard titration solution of perchloric acid until the solution changes from purple to green for 15s without changing color as the end point.
2.8 Record the volume of standard solution consumed.
2.9 Carry out the blank test at the same time.
3. Calculation and results
The free amino acid content X in the reagent is expressed as a mass fraction (%), calculated according to formula (1): X=C×(V1-V0) ×0.1445/M×100%...... .......(1)
In the formula: C - concentration of standard perchloric acid solution in moles per liter (mol/L)
V1 - Volume used for titration of samples with standard perchloric acid solution, in milliliters (mL).
Vo - Volume used for titration blank with standard perchloric acid solution, in milliliters (mL);
M - Mass of the sample, in grams (g ).
0.1445 - Average mass of amino acids equivalent to 1.00 mL of standard perchloric acid solution [c (HClO4) = 1.000 mol / L].
4. Calculation of chelation rate
The chelation rate of the sample is expressed as mass fraction (%), calculated according to formula (2): chelation rate = (total amino acid content - free amino acid content)/total amino acid content×100%.
Post time: Sep-17-2025
